Preliminary phytochemical screening of the ethanolic seed extract of Hibiscus sabdariffa l. revealed the presence of alkaloids, saponins, tannins, anthraquinones, steroids, cardiac glycosides, flavonoids and phlobatanins. The acute toxicity LD50 of Hibiscussabdariffa l. seedextract in albino rats was found to be above 5000 mgkg-1. The lactogenic effect of ethanolic seed extract of Hibiscus sabdariffa l. was investigated by administering extract and metoclopramide in albino rats. The extracts were administered at varying doses (200, 400, 800 and 1600mgkg-1) and drug (5mgkg-1) for six days orally. There was significant increase (p<.01) in serum prolactin levels in a dose dependent manner. The doses of 800mgkg-1 and 1600mgkg -1 seems to be more effective with serum prolactin levels of 15.74±0.8 and 17±0.6 respectively. The serum prolactin level of metoclopramide (17.50ng/ml) when compared to the effective dose showed no significant difference (P>0.05). The ethanolic seed extract of Hibiscus sabdariffa l. possess lactogenic effect. Keywords: Hibiscus sabdariffa | prolactin | lactation | seed extract | milk.